Mucin topical deodorants and antiperspirants

ABSTRACT

The present invention relates to aluminum-containing and aluminum-free mucin topical deodorants and antiperspirants. It is an objective of this invention to provide a topical deodorant and/or antiperspirant formulation for suppressing emergence of body odor formation, comprising a mucin and a physiologically acceptable carrier, which is particularly free of aluminum-containing substances. It is a further objective of this invention to provide the use of a mucin in the preparation of a topical deodorant and/or antiperspirant formulation for suppressing emergence of body odor formation, said formulation being particularly free of aluminum-containing substances.

FIELD OF THE INVENTION

The present invention is comprised within the field of cosmetic andpersonal care industries, particularly deodorants and antiperspirantsfor the human body.

BACKGROUND OF THE INVENTION

Deodorants:

A deodorant is a substance that is used to eliminate bad odor which isgenerally caused by sweating. Odor is generated from secretions of theapocrine sweat glands, which are primarily contaminated by Coryneformand Staphylococcus bacteria, as for example C. jeikeium, C. striatum, C.xerosis, S. epidermis and S. haemolyticus. C3-fatty acids, (iso)butyricacid, isovaleric acid, and androgen steroids have been exemplified asthe odorous substances. Deodorants are mostly applied locally on surfaceof the body. Many chemical compounds are used to make a deodorant, whichcan also contain antiseptics or specific agents that destroy or preventbacteria, which is responsible for metabolizing proteins and fattyacids.

One of the utmost advantages of deodorants is that it has the capabilityto manage the odor by neutralizing it. A deodorant has that ability toprotect the body from excessive sweat, and eliminates bacteria thatcauses bad odor. Deodorants are preferred more than antiperspirants asit has proved a fact that deodorants do not prevent sweatingunnaturally. There are different kinds of deodorants containing scentsfrom floral, sporty and the breezy ones. Besides these choices, thereare some deodorants which have no scents but works perfectly inneutralizing the awful odor. People can even try making their own homemade deodorants by using natural ingredients that pamper the skin andgive a feeling of freshness throughout the day.

Deodorants are used to control the body odor, but when you usedeodorants, some of them have side-effects on clothes and skin. Theseside effects are for example decreases in perspiration:aluminum-containing deodorants block sweat ducts (sudoriparous ducts),and this can build up toxins in our armpits. Aluminum-containingsubstances also can buildup estrogen, which causes breast cancer.

Antiperspirants:

An antiperspirant is a substance which is used to prevent or reducesweating through blocking the pores by using for example aluminum,effectively changing the function of the body. Without sweat, thebacteria cannot metabolize proteins and fatty acids that cause bodyodor. Antiperspirants have some side-effects like: rashes and skinirritations but they can be easily treated—the use of antiperspirants onbroken skin is not advisable as they can cause blood poisoning.

Antiperspirants have a certain fragrance, which has a capability toprevent sweat and to neutralize the unpleasant odor. Antiperspirantsblock the pores with the help of chemical substances, thus controllingsweating. Antiperspirants can contain aluminum, which helps clogging thepores and it also prevents the sweat spots. Antiperspirants usually havetheir effects for 12 or 48 hours. However, the use of excessiveantiperspirants can also cause complications and, therefore, it isadvised that antiperspirants are used sparsely.

It is known in the art that antiperspirants are used for controllingsweat. However, there are some side effects that people must be awareof, as for example lumps: some antiperspirants block the pore of theskin and cause bacterial infections like armpits' lumps.Aluminum-containing antiperspirants can also cause breast cancer.

Currently, there are deodorants combined with antiperspirants, andvice-versa.

Aluminum:

Most aluminum compounds currently used in cosmetics are exemplified asfollows: aluminium starch octenylsuccinate, aluminum chlorohydrate,aluminum hydroxide, aluminum chloride and aluminum-zirconium compounds.

Aluminum hydroxide is used widely in antiperspirants, and as a filler incosmetics. Aluminum starch octenylsuccinate is used in cosmeticformulations as anti-caking and viscosity-increasing agents. Aluminumchloride, aluminum chlorohydrate and aluminum-zirconium compounds, mostnotably aluminum zirconium tetrachlorohydrex gly and aluminum zirconiumtrichlorohydrex gly, are the most widely used in antiperspirants.

Aluminum salts can account for 25% of the volume of someantiperspirants, and common sources of aluminum exposure for humans showthat antiperspirant use can significantly increase the amount ofaluminum absorbed by the human body. It is known that, after a singleunderarm application of antiperspirant, about 0.012% of aluminum isabsorbed by the body.

At high doses, aluminum itself adversely affects the blood-brainbarrier, is capable of causing DNA damage, and has adverse epigeneticeffects. The absorption of aluminum through the skin can cause a greaterburden on the body than oral ingestion. After using aluminum-containingantiperspirants, in case of aluminum absorption by the body, it is knownthat aluminum can still be present in the blood 15 days after oneapplication of said aluminum-containing antiperspirants to the armpit.Consequently, applying aluminum to the skin is a very effective way toget aluminum in the human blood system, and the brain. Aluminum speciesused in cosmetics can cause a series of diseases and disorder, as forexample pulmonary irritation and toxicity, conjunctivitis and purulentophthalmitis, mild eye irritation, breast cancer, renal dysfunction,Alzheimer's disease, skin irritation, among others.

Mucins:

Mucins correspond to glycoproteins present in animals and microbes asthe main component of mucus. They represent complex substances havingheterogeneous characteristics in biological and medical sciences. Theirstructural properties including the full-length sequence of their mainpeptide chain and the composition and structure of their branched glycanchains have been clarified only partially. Mucins act as a protectinglining of the mucosa surface, moisturizing material, antimicrobialreagent, lubricant, surfactant, reducer of surface tension, coatingmaterial, antifreeze matrix, ion-exchange polymer, amongst otheractivities (Ushida, K. & Murata, T. “Chapter 4: Materials Science andEngineering of Mucin: A New Aspect of Mucin Chemistry” v. 39, p.115-159, 2013).

A number of recent research studies based on glycoscience have clearlyproved the ability of mucins to perform molecular recognition via theirglycan chains, which play the main role in various activities occurringin mucus and around cell surfaces. Typical ligands for glycan chains inmucins are those of the lectin family. This molecular recognitionproperty is the reason for the various functions of mucins mentionedabove. Mucins are utilized as a group of efficient materials forcontrolling the above-mentioned ubiquitous but unique bioactivities ofmucins in medical, hygiene, pharmacological, and industrial applications(Ushida & Murata, 2013).

Until 2013, about 20 human mucins have been identified using seriesnames with the header MUC followed by a number. Each MUC is identifiedin a gene by cDNA cloning with a specific amino acid sequence of themain peptide chain. All of the listed MUC series are roughly separatedinto two groups: (A) membrane-bound (cell surface) mucins; and (B)secreted (airway) mucins. Examples of membrane-bound (cell surface)mucins are MUC1 and MUC3A. Examples of secreted (airway) mucins areMUC2, MUC5AC, MUC5B, MUC6, MUC8 and MUC19 (gel forming), and MUC7 andMUC9 (nonpolymeric). Each type of MUC is secreted throughout the variousmucosal surfaces in the human body. For example, MUC5AC is abundant inboth gastric and lachrymal fluids (Ushida & Murata, 2013).

Mucins extracted from other mammals have also been investigated for along time. They are abundant as commercial materials with relativelyreasonable prices. Gastric mucins from pig (porcine gastric mucin, PGM)and rat (RGM), and submaxillary gland mucins from pig (porcinesubmaxillary gland mucin, PSM), cow (bovine submaxillary gland mucin,BSM), sheep (ovine submaxillary gland mucin, OSM), mouse (MSM), and rat(RSM) are commonly used (Ushida & Murata, 2013).

Glycosylated Polypeptides:

It is known in the art that, depending on the processes and techniquesto be applied, the handling and use of mucin can result in the proteicdigestion and fragmentation thereof. For instance, during the extractionprocess of mucine, pepsin can be used. However, said pepsin usuallydigests and fragmentizes the proteic backbone of full macromolecule ofmucin, being obtained as active ingredients glycosylated polypeptides.The state of the art teaches that glycosylated polypeptides fromdigested and fragmentized mucin are obtained, which show activity forinhibiting microbial biofilm from Pseudomonas aeruginosa (Haley, C. L.et al. “Mucin inhibits Pseudomonas aeruginosa biofilm formation bysignificantly enhancing twitching motility” Can J Microbiol; 60(3):155-166; March 2014).

STATE OF THE ART

The use of mucin in cosmetic topical products for retaining moisture inthe skin is described in the state of the art as, for example, inJapanese patent applications JP 62153206 A (published on Jul. 8, 1987,in the name of Kanebo Ltd.), JP 63041412 A (published on Feb. 22, 1988,in the name of Kanebo), JP 03287510 A (published on Dec. 18, 1991, inthe name of Pola Chemical Industries and Teikoku Hormone MFG) and JP10182408 A (published on Jul. 7, 1998, in the name of Kose). However,said prior art references are not focused on deodorant andantiperspirant cosmetics and their effects on the human body and,although aluminum salts are not disclosed therein, said documents do notdescribe or suggest the benefits and advantages of aluminum-freedeodorants and antiperspirants containing mucin.

US patent application US 2015/030661 A1 (published on Jan. 29, 2015, inthe name of Massachusetts Institute of Technology) teaches a multilayerfilm comprising alternating layers of a glycosylated polymer and alectin, wherein the lectin crosslinks the glycosylated polymers, saidglycosylated polymer can be a mucin selected from the group consistingof porcine gastric mucin (purified porcine gastric mucin composedprimarily of MUC5AC, MUC2, MUC5B, and MUC6), bovine submaxillary mucin(BSM) or a combination thereof. The multilayer film of US 2015/030661can be lectin depleted. However, said prior art reference was developedfor pharmaceutical purposes, and it is not focused on deodorant andantiperspirant cosmetics and their effects on the human body and,although aluminum salts are not disclosed therein, said documents do notdescribe or suggest the benefits and advantages of aluminum-freedeodorants and antiperspirants containing mucin. US 2015/030661 A1addresses the ability of the multilayer film to act on the formation ofmicrobial biofilm. In contrast, US 2015/030661 A1 is different from thepresent invention because it does not use the mucin alone for evaluationof effectiveness to antiperspirant and deodorant activities. Other thanthat, said prior art document aims at testing glycosylated polymers asmucin in combination with lecithin having main application in the formof a multilayer film acting as a delivery system of active ingredients.

United States patent application US 2004/180027 A1, which was publishedon Sep. 16, 2004 in the name of Genencor International, Inc., provides apersonal care composition comprising an effective amount of a repeatsequence protein polymer and a physiologically acceptable carrier orexcipient, wherein said repeat sequence protein polymer comprises arepeating amino acid sequence unit derived from mucin or others. Thecomposition of US 2004/180027 A1 can be used as an antiperspirant.However, said US document describes that the repeat sequence proteinpolymers used therein are advantageous in providing personal careproducts when modified with desired chemical agents, as for examplealuminum-containing antiperspirant actives. US 2004/180027 A1 differsfrom the present invention because it does not describe or suggest amucin polymer in the form of a full glycoprotein or a mixture ofglycosylated polypeptides from mucin. Said prior art document uses apolymer obtained in an amount of repetition sequences of amino acidsfrom a protein polymer, as for example mucin, and for this reason ittests a structure formed by the same amino acid sequence and not by allamino acids comprised by the mucin, being therefore chemically andcompletely different macromolecules.

International publication WO 2014/055127 (published on Apr. 10, 2014, inthe name of Katharina Ribbeck) refers to a method of inhibitingvirulence of one or more microorganisms, and/or inhibiting one or moremicroorganisms from attaching to a surface, forming suspended aggregatesor a combination thereof, comprising contacting the one or moremicroorganisms, the surface, or a combination thereof with purified,native, non-human mucin, wherein said non-human mucin can be porcinegastric mucin particularly comprising MUC5AC, MUC2, MUC5B MUC6 orcombinations thereof, and wherein said one or more microorganisms can beone or more bacteria, archaea, fungi or a combination thereof. Noaluminum salt is used in said WO document. However, although aluminumsalts are not disclosed therein, said prior art reference does notdescribe the benefits and advantages of aluminum-free deodorants andantiperspirants containing mucin nor even the benefit of using mucins inplace of aluminum salts in deodorant compositions. Further, there is nodescription or suggestion in WO 2014/055127 indicating that its objectis directed to the axillary microorganisms approached by the presentinvention. This technical feature is relevant, as it is known in the artthat biofilm formation has significant particularities among thedifferent species of microorganisms. Moreover, according to thisinvention, the matrix tested in the in vivo panel is an organic matrix,unlike inorganic matrices as are the culture plates. The effectivenessof mucin in view of axillary matrix also brings technical features ofspecificity and differentiation for the present invention, which so farhave not been obtained in the state of the art.

Thus, it is desirable to provide deodorant and antiperspirant cosmeticproducts comprising mucin. Particularly, said deodorant andantiperspirant cosmetic products comprising mucin are free ofaluminum-containing substances.

SUMMARY OF THE INVENTION

The present invention relates to aluminum-containing and aluminum-freemucin topical deodorants and antiperspirants. It is an objective of thisinvention to provide a topical deodorant and/or antiperspirantformulation for suppressing emergence of body odor formation, comprisinga mucin and a physiologically acceptable carrier, which is particularlyfree of aluminum-containing substances. It is a further objective ofthis invention to provide the use of a mucin in the preparation of atopical deodorant and/or antiperspirant formulation for suppressingemergence of body odor formation, said formulation being particularlyfree of aluminum-containing substances.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows mucins that can prevent bacteria from forming biofilms,which are able to prevent the formation of Pseudomonas aeruginosabiofilms on surfaces and maintain the bacteria in the planktonic state.

DETAILED DESCRIPTION OF THE INVENTION

A thick, well-hydrated coat of mucus fully covering all moist epitheliain the human body is the key to ensure proper lubrication and protectionagainst pathogens. For example, the inventors verified that healthymucins, arranged as a 3D gel, can suppress a range of virulence traitsacross several microbial species, showing mucins have powerfulcapabilities of regulating microbial behavior. For example, mucins canprevent Pseudomonas aeruginosa from colonizing a surface and fromforming potentially deleterious biofilms. Moreover, mucins can preventthe yeast Candida albicans from adhering to an underlying substrate, andmoreover from switching from the benign and non-infectious yeast forminto the potentially pathogenic filamentous form.

One advantageous feature of mucins is that they suppress microbialvirulence without killing the microbes, implying that their presencewill not select for the emergence of resistance. Hence, mucins are idealnatural components for using within cleansing, hygiene and cosmeticproducts that aim at preventing microbial infections or regulatingmicrobial pathogenicity without altering the naturally complexmicroflora of the human body's surfaces. The inventors of the presentinvention researched and found that the use of mucins is of high valuein products that regulate bacterial populations or prevent the formationof pathogenic biofilms for topical treatments.

The mucus barrier is a vital part of our body, and any disturbance inits function can result in an increased susceptibility to pathogens.Indeed, several important diseases such as cystic fibrosis, inflammatorybowel diseases and dry eye syndrome are correlated with a defectivemucus barrier. Decrease in hydration and protective efficiency is alsocommonly noticed with oral diseases. The underlying reasons for this aremostly unknown, but are very likely to be a result of both insufficienthydration and poor mucus production.

One object of the present invention is to develop novel andnature-inspired strategies for suppressing the emergence of body odorformation. The underarm pit plays an important role in the generation ofbody odor. Odor is generated from secretions of the apocrine sweatglands, which are primarily contaminated by Coryneform andStaphylococcus bacteria. One potential strategy for suppressing bodyodor formation is to apply topically to the armpit hydrated mucinpolymers that can suppress a range of microbial virulence traits,including the colonization of an underlying epithelium and biofilmformation, as well as favor growth of beneficial microbes that stabilizethe microflora in the armpit.

The inventors researched that natively purified mucins can efficientlysuppress biofilm formation and other virulence factors in a range ofmicrobes, including Pseudomonas aeruginosa, E. coli, Candida albicans,and Streptococcus mutans. They found that native mucins will also limitthe virulence phenotypes in microbes responsible for odor formation byCoryneform and Staphylococcus bacteria. One specific goal of thisinvention is to demonstrate the behavior of the selected microbes C.jeikeium, C. striatum and S. haemolyticus in a mucin environment. Theseorganisms colonize the underarm epithelium and can cause body odorformation. Specifically, the present invention 1) characterized theinfluence of native mucins on the behavior of these selected microbes,and 2) studied the influence of mucins on multispecies interactionsbetween bacteria that are responsible for the formation of bad odor andantagonistic bacteria that can suppress their growth.

As described above, depending on the handling and use of mucin,digestion and fragmentation of the proteic backbone of fullmacromolecule of mucin can occur. Thus, glycosylated polypeptidesderived from said digested and fragmentized mucin can be obtained asactive ingredients. According to the present invention, saidglycosylated polypeptides present cosmetic activities, particularlydeodorant and/or antiperspirant activities. For the purposes of thisinvention, the mucins used herein can be selected from full mucinmacromolecules, digested and fragmentized mucins (glycosylatedpolypeptides therefrom) and combinations thereof.

The mucin used in the present invention prevents or inhibits theadhesion of microorganisms to underlying surfaces, in order to targetmicroorganisms' virulence and to suppress same. In the human body, mucinis employed for suppressing body odor formation that occurs as a resultof suppressing a range of microbial virulence traits. Saidmicroorganisms can be bacteria, archaea, fungi or a combination thereof.

For the purposes of the present invention, mucin is selected from thegroup consisting of porcine gastric mucins (PGM), porcine submaxillarygland mucins (PSM), rat gastric mucins (RGM), bovine submaxillary glandmucins (BSM), ovine submaxillary gland mucins (OSM), mouse submaxillarygland mucins (MSM), rat submaxillary gland mucins (RSM), purified nativemucin, hydrated mucin polymer, mucin obtained or derived from fish, andmixtures thereof. Particularly, said mucin is mucin type II (or MUC TypeII), mucin type III (or MUC Type III) MUC5AC, purified native mucin, PGMType II, PGM Type III, Sigma Mucin Type II, Sigma Mucin Type III,hagfish slime mucin, and mixtures thereof.

The present invention is particularly free of aluminum-containingsubstances. Another object of the present invention is to provide adeodorant and/or antiperspirant that, in addition to comprising mucin,it does not contain any aluminum species. This particular technicalfeature (absence of aluminum-containing substances) aims at avoiding thedamages and disadvantages resulted from the use of aluminum through thebody, thus combining the benefits of the mucin active with theprevention of harmful aluminum substances.

It is an embodiment of this invention a topical deodorant and/orantiperspirant formulation for suppressing emergence of body odorformation, comprising a mucin and a physiologically acceptable carrier,which is particularly free of aluminum-containing substances.Particularly, said mucin is present in an amount of 0.5 to 15% by weightof the formulation.

It is a further embodiment of this invention the use of a mucin in thepreparation of a topical deodorant and/or antiperspirant formulation forsuppressing emergence of body odor formation, said formulation beingparticularly free of aluminum-containing substances.

The formulations envisaged by the present invention can compriseadditional components regularly used in the cosmetic field, being asnon-limitative examples: water, perfumes, fragrances, vegetable oils,vegetable essences, sunscreens, emollients, moisturizers, preservatives,surfactants, pH modifiers, vitamins, emulsifiers, lubricants, viscositymodifiers, antioxidants, among others.

Said invention's formulations can be presented as a wash, lotion, cream,emulsion, gel, soap, roll-on, stick, aerosol, and spray to be applied tothe body.

EXAMPLES Example 1—Determination of the Effects of Mucins on SelectedIndividual Odor-Forming Bacterial Species

Previous studies that address the effects of mucins on S. mutansbehavior used commercially available pig gastric mucins, which differfrom native mucins in important ways. Most importantly industrialpurification leads to degradation of both the protein backbone andmucin-associated glycans, rendering the molecules non-functional inseveral ways. The inventors evaluated the effect of mucins on bacterialphysiology using purified native mucins from various sources (FIG. 1).

a) Growth Rate in Native and Sigma Aldrich Mucins:

The inventors identified the influence of native mucins on the growth ofC. jeikeium, C. striatum and S. haemolyticus in nutrient rich orchemically defined culture media. Growth experiments in nutrient richmedia with mucins revealed if native mucins are detrimental to microbialgrowth. Growth experiments in chemically defined media supplemented withmucins revealed if the microbes can utilize mucins as a nutrient source.A disc diffusion assay was performed to determine the MIC of mucins.

b) Odor Formation and Biofilm Formation in Native Mucins and SigmaAldrich Mucins Type II:

Biofilm formation contributes to malodor formation and, by analogy, itis likely also involved in odor formation in the armpit. Data obtainedby the inventors shown that mucins behave as a defense system to protectthe surfaces from colonization by P. aeruginosa, Candida albicans, andS. mutans, and it was found that these biopolymers are also effectiveagainst surface colonization of C. jeikeium, C. striatum, C. xerosis andS. haemolyticus. It was investigated surface attachment and biofilmformation in the presence of mucins. Static biofilms and flow-cellbiofilms were grown in the presence or absence of mucins. If mucins caninhibit biofilm formation, there is potential to use this biopolymer informulations that aim to suppress odor formation. According to thisinvention, mucins are able to adsorb and neutralize secreted smallmolecules that contribute to odor formation.

The observed timeline was:

-   -   Growth rate: 2 months    -   Surface attachment: 3 months    -   Biofilm formation: 6 months

The types of mucin that were tested are MUC type II (native) fromporcine gastric tissue or from hagfish slime and MUC type III from SigmaAldrich.

Example 2—In Vitro Assay

The protocol described in Haley et al. (2014) was also used in thisexample. The test for biofilm formation with 3 different microorganisms(Staphylococcus haemolyticus, Corynebacterium striatum andCorynebacterium xerosis) was performed as follows: control assay withoutapplication of the sample, carrying out readings in times of 8, 24 and48 hours for each microorganism, in triplicate; assay with two separatesamples applied at zero time with microorganisms for verification ofbiofilm formation with the product applied, carrying out readings intimes of 8, 24 and 48 samples, in triplicate. At the same time, it wasconducted a test for minimum inhibitory concentration, in duplicate,with 3 microorganisms and 2 products.

Example 3—In Vivo Assay

It was performed a test for effectiveness regarding odor reduction andmodulation of microbiota of the axilla, over 25 volunteers, using aproduct without antiperspirant and another product havingantiperspirant. The metagenomics lasted 4 months, and it was dividedinto 3 stages: 1) DNA extraction and sample preparation (1 month); 2)sequencing and bioinformatics (2 months); 3) data analysis andpreparation of the report (1 month). There were generated results of thequalitative and quantitative identification of axillary microbiota inconditions before and after application of the test products, andanalysis' data of the relationship between qualitative and quantitativeresults of the microbiota with the mal odor phenotype.

Example 4—Cream Formulation (Consistent Emulsion)

Phase Cream (consistent emulsion) % 1 Aqua or water 47.6 1 Disodium EDTA0.1 2 Hydroxypropyl startch phosphate 2 3 Ceresin 3 4 Cetearyl alcohol7.5 4 Ceteareth20 1.75 4 Dicaprylyl carbonate 1 4 Olus oil/algae oil 1 4BHT 0.05 5 2-methyl-5-cyclohexylpentanol 0.4 6 Mucin 0.5-15 7 Talc 2 8Cyclopentasiloxane and dimethiconol 1 8 Cyclopentasiloxane 1 9 DMDMhydantoin 0.6 10 Fragrancia cotton glaze CL 2 1

Example 5—Roll-on Formulation (Fluid Emulsion)

Phase Roll-on (fluid emulsion) % 1 Aqua or water 53.8 1 Disodium EDTA0.1 2 Hydroxypropyl started phosphate 2 3 PPG15 stearyl ether 1 3Steareth 2 3 3 Steareth 21 1.1 3 BHT 0.05 3 Olus oil/algae oil 3.8 4Silica dimethyl silylate 0.15 4 2-methyl-5-cyclohexylpentanol 0.4 5Mucin 0.5-15 6 DMDM hydantoin 0.6 7 Parfum 1 4 PPG20 methyl glucoseether 3

Example 6—Aerosol Formulation

Aerosol Phase BIP 1 PPG14 butyl ether 1 Disterardimonium hectorite 1Propylene carbonate 2 Cyclopentasiloxane 2 C12-15 alkyl benzoate 2Caprylyl methicone 3 Mucin 4 Cyclopentasiloxane (and) cetearyldimethicone/vinyl crosspolymer 5 Olus oil 6 BHT 72-methyl-5-cyclohexylpentano

Example 7—Spray Formulation

Phase Spray % 1 Alcohol 70.545 2 BHT 0.05 2 Cosmocil 0.3 2 Denatoniumbenzoate 0.005 3 Zemea 2 5 Lactic acid 0.1 5 Aqua (or) water 25 6Fragrancia lovely woman body oil 2 7 Mucin 0.5-15

Example 8—Roll-on Formulation

Ingredients % 1 AQUA 57.3 1 Disodium EDTA 0.1 2 Hydroxypropyl StartedPhosphate 1.5 3 STEARETH 21 1.1 3 STEARETH 2 3 3 BHT 0.05 3 PPG-15STEARYL ETHER 1 3 Olus Oil 3.8 4 2-METHYL 5-CYCLOHEXYLPENTANOL 0.4 4Silica Dimethyl Silylate 0.15 5 MUCIN 0.5-15 6 DMDM hydantoin 0.6 6Parfum 1

Example 9—Deo Cream Formulation

Ingredients % 1 AQUA 47.6 1 Disodium EDTA 0.1 2 Hydroxypropyl StartchPhosphate 2 3 Ceresin 3 4 Cetearyl Alcohol 7.5 4 Ceteareth-20 1.75 4Dicaprylyl Carbonate 1 4 Olus Oil 1 4 BHT 0.05 5 2-METHYL5-CYCLOHEXYLPENTANOL 0.4 6 MUCIN 0.5-15 7 Talc 2 8 CYCLOPENTASILOXANEAND 1 DIMETHICONOL 8 Cyclopentasiloxane 1 9 DMDM hydantoin 0.6 10 Parfum1

Example 10—Aerosol Formulation

Ingredients % Fase 21639 (30%) 1 PPG-14 Butyl ether 13.33 1 BENTONE 38 VCG_DISTEARDIMONIUM 2.17 HECTORITE_ELEMENTIS 1 Propylene Carbonate 0.73 2Cyclopentasiloxane 20.77 2 C12-15 Alkyl Benzoate 14.17 2 CaprylylMethicone 4.17 3 MUCIN 0.5-15 4 Cyclopentasiloxane (and) CetearylDimethicone/Vinyl 4.17 Crosspolymer 5 Olus Oil 2.33 6 BHT 0.17 6 Parfum3.33 7 2-Methyl 5-Cyclohexylpentanol 1.33 Fase 21642 (70%) 1BUTANE_ISOBUTANE_PROPANE_70_30 100

Example 11—Moisturizing Formulation (for Body)

Ingredients % 1 Butyrospermum Parkii (Shea) Butter 1 1 Caprylic/Caprictriglyceride 2 1 Canola Oil 2 2 Dicaprylyl Ether 2 2 ISOHEXADECANO 2 2Ricinus Communis Seed Oil 2 2 Tocopheryl Acetate 0.1 2 Glycerin 5 2 OlusOil 2 2 Sphingoceryl WS LS 9859 0.2 3 Xanthan Gum 0.2 3 SodiumPolyacrylate 0.9 4 Eumulgade CM 2 5 AQUA 76.1 5 Disodium EDTA 0.1 6Methylisothiazolinone 0.1 6 DMDM hydantoin 0.5 6 LAMESOFT TM BENZ 0.8 7MUCIN 0.5-15 7 Parfum 0.5 8 ARISTOFLEX AVL 0.4

Example 12—Moisturizing Formulation (for Feet)

Ingredients % 1 AQUA 46.2 2 Disodium EDTA 0.1 3 Xanthan Gum 0.1 3 SodiumPolyacrylate 0.5 4 Cetyl Lactate and Cetyl Alcohol 2 4 GlycerylStearate/Glyceryl Distearate 2 4 Butyrospermum Parkii (Shea) Butter 10 4Caprylic/Capric triglyceride 1 4 Canola Oil 2 4 Olus Oil 1 4 Manteiga deCacau Refinada de UIB 0.3 5 AQUA (OR) WATER 26 5 Glycerin 5 6 EumulgadeCM 1.5 7 CYCLOPENTASILOXANE AND DIMETHICONOL 1 8 MUCIN 0.5-15 9Methylisothiazolinone 0.1 9 DMDM hydantoin 0.5 10 Tocopheryl Acetate 0.111 FRAGRÂNCIA LAVMILK BODY MOD13B1C - 0.5 238730

Example 13—Spray Formulation

Ingredients % 1 Alcohol 71.5 1 MUCIN 0.5-15 1 BHT 0.05 1 BENZOPHENONE-20.05 1 Denatonium Benzoate 0.005 1 CI 42090 0.0207 1 CI 60730 0.135 2PARFUM 3 3 Propanediol 1 4 AQUA 23.9393

Example 14—Cologne Formulation

Ingredients % 1 Alcohol 77.329 1 MUCIN 0.5-15 1 BHT 0.05 1BENZOPHENONE-2 0.05 1 Denatonium Benzoate 0.005 2 Parfum 10 3 AQUA 12 4SOL ALCOOL 70% CORANTE VERDE (NATURA) 0.025 ORGANICO 4 SOL. CORANTE 0.1%VIOLETA PURICOLOR 70% 0.212 ÁLCOOL 4 SOLUÇÃO CORANTE RED 4 0.1% - ÁLCOOL70° 0.229

1. A topical deodorant and/or antiperspirant formulation for suppressingemergence of body odor formation, comprising a mucin and aphysiologically acceptable carrier, which formulation is particularlyfree of aluminum-containing substances.
 2. The formulation according toclaim 1, wherein said mucin is present in an amount of 0.5 to 15 wt %.3. Use of a mucin in the preparation of a topical deodorant and/orantiperspirant formulation for suppressing emergence of body odorformation, said formulation being free of aluminum-containingsubstances.
 4. The formulation according to claim 1, wherein the mucinis selected from full mucin macromolecules, digested and fragmentizedmucins (glycosylated polypeptides therefrom) and combinations thereof.5. The formulation or use according to claim 1, wherein the mucin isselected from the group consisting of porcine gastric mucins (PGM),porcine submaxillary gland mucins (PSM), rat gastric mucins (RGM),bovine submaxillary gland mucins (BSM), ovine submaxillary gland mucins(OSM), mouse submaxillary gland mucins (MSM), rat submaxillary glandmucins (RSM), purified native mucin, hydrated mucin polymer, mucinobtained or derived from fish, and mixtures thereof, preferably mucintype II (or MUC Type II), mucin type III (or MUC Type III) MUC5AC,purified native mucin, PGM Type II, PGM Type III, Sigma Mucin Type II,Sigma Mucin Type III, hagfish slime mucin, and mixtures thereof.
 6. Theformulation or use according to claim 5, wherein the mucin is MUC TypeII, MUC5AC, purified native mucin, and mixtures thereof.
 7. Theformulation or use according to claim 1, wherein the suppress of bodyodor formation occurs as a result of suppress a range of microbialvirulence traits.
 8. The formulation or use according to claim 1,wherein it further comprises one or more cosmetically acceptablecomponents selected from the group consisting of water, perfumes,fragrances, vegetable oils, vegetable essences, sunscreens, emollients,moisturizers, preservatives, surfactants, pH modifiers, vitamins,emulsifiers, and lubricants.
 9. The formulation or use according toclaim 1, wherein it is in the form of a body wash, lotion, cream,emulsion, gel, soap, roll-on, stick, aerosol, or spray.